QC收费标准

昆泰锐QC收费标准

对于您近期对于我们收费情况的疑惑,我们给您做一个详细的解疑:

1. 常规样品,即样品质量无问题,引物质量无问题的样品,有效读长达到800bp,即将收费。

2. 样品存在特殊结构的,如发卡结构,poly结构,重复序列等结构,导致反应信号衰减较快,反应读长较短的或者结构之后出现mix套峰的,将收费。
       发卡结构,存在较大和复杂的空间位阻,所以测序反应进行到此处时,容易出现信号中断;Poly结构和重复序列,本身会形成二级结构,且Poly结构和重复序列在测序进行时,容易出现读取碱基时出现碱基位移,所以该结构之后会出现套峰。

3. 样品本身质量有问题,如size较大,浓度太低,导致反应信号低,读长较短的,读长达到了500bp,将收费。

4. 引物本身有问题的,如引物二级结构较多,导致测序结果反应信号很低,将收费。
       较多的引物二级结构,从测序结果上反映就是在原始反应信号最前面会出现较高的引物二聚体峰,较多的引物二级结构会导致样品在开始测序反应前,就会被引物二聚体消耗掉很多的酶和试剂,这样可能会导致样品在后续反应总由于酶的消耗,不足以维持后续反应,而出现反应信号很低,读长较短。

5. 样品质量没有问题,反应信号值理想,出现mix套峰的,将收费。
       DNA模板上出现二处以上的引物结合位点(多引物结合位点),或者DNA模板上有严重的重复序列,以及测序引物不纯时, 测序结果便会出现套峰现象;另外,PCR样品或者克隆样品出现碱基点突变或者缺失和插入突变等情况,测序结果均会出现mix套峰。

6. 对于出现测序结果OS(反应信号值太高),LR(胶分辨率太低),DI(毛细管电泳出现延迟进样)的情况,我们将不收费,并重新调整实验再测序,调整后测序成功的再收费。

7.对测序结果存在质疑的反应,需明确指出质疑的位置,若质疑区在可信区(100-700bp)之内,且无法判断的情况,我们将提供一次免费调整实验,若调整实验与第一次结果一致,将收取一次测序费用。

DNA Sequencing

Sample Preparation Instructions

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

Benefits

  • The most flexible sequencing service with the highest success rate
  • Skilled interpretation of your sequencing results
  • Trouble-shooting based on our expertise and our proposals to deal with sequencing difficulties (hairpin, GC-rich templates)
  • Additional services (template amplification, PCR purification) are included in the service package
  • Optional sequence editings and sequence alignment are available free of charge

Quality

  • Average reading lengths: 700-1000 bases.
  • Color printouts of chromatographs are provided free of charge upon request.
  • Optional sequence alignment saves time when analyzing sequencing results.
  • Sequence editings are provided upon request. Sequence editing means a manual proofread performed by an experienced staff to correct mistakes /uncertainities in sequences generated by the automatic base callor.
  • Trouble shooting: We propose further trouble shooting steps based on the sequencing results. However, it is your decision whether we continue or stop the sequencing job.
  • Repeat policy: Failed samples will be repeated based on our interpretation of your sequencing results, and the repeat is free of charge.

Turnaround

The general turnaround time is 24 hours. Add another 24 hours for E.coli strains.

Colony PCR

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

This protocol is designed to quickly screen for plasmid inserts directly from E. coli colonies. The plasmid should be high copy number such as pUC18 pUC 19, or pBluescript, etc. Even though blue/white screening can be used to determine if inserts are present, this technique can be used to determine insert size and/or orientation in the vector. We have an optimized colony PCR protocol to tailor your insert size and plasmid type. After colony PCR is done, a small amount of product will be checked by by agarose gel electrophoresis. If predicted band is observed, ExoSAP process will be carried out to remove excess primer and dNTP, and then DNA sequencing will be executed.

Template Amplification (RCA)

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

Template amplification uses bacteriophage Phi29 DNA polymerase to in vitro amplify plasmid DNA, also called rolling circle amplification (RCA). RCA has a strong preference of circular DNA templates like plasmid DNA. Plasmid amplified by RCA is suitable for DNA sequencing. You can submit DNA samples in case you have limited DNA samples, or E.coli strains. Turnaround time for RCA amplification is 24 hours. When combined with DNA sequencing, turnaround time for the whole process is 48 hours.

Nucleic Acid Extraction

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

Plasmid preparation

A plasmid preparation is a method used to extract and purify plasmid DNA. We used Qiagen Miniprep to Maxiprep kits to purify plasmid DNA for your need. We accept various forms of samples, such as bacteria culture, bacteria colony plate, bacteria seed or ligation product. After plasmid preparation is done, DNA sequencing will be preformed. Plasmid DNA or bacteria seed can be returned to you upon request.

Genomic DNA preparation from tissue

A customized protocol is available for extracting genomic DNA from animal tissue (commonly mouse tail). This yields DNA suitable most downstream applications, such as genotyping, Southern blotting, sequencing, etc. The genomic DNA can be returned to you upon request.

Genotyping

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

Genotyping refers to the process of determining the genotype of an individual by the use of biological assays. Our methods of doing this include PCR, DNA sequencing and qPCR. The technology is important in clinical research for the investigation of disease-associated genes.

Genotyping applies to a broad range of research interests including SNP detection, transgenic/knockout determination and strain determination.

Cosmid and BAC Sequencing

Reactions

Sequencing of cosmid and Bac-DNA is similar to sequencing of plasmid templates except that more DNA is needed. 2 ug Cosmid/Bac DNA is needed for direct sequencing. There is no additional charge for Cosmid/Bac sequencing.

We also offer the whole cosmid- and Bac-DNA shotgun sequencing. Please inquire about details.

High Throughput Sequencing Services

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

Reactions

Single-pass sequencing reactions are performed in 96-well PCR plates.

Sample Submission

  • The DNA samples and the primers should be pre-mixed in defined volume.
  • Samples must be submitted in 96-well PCR plate format to preserve samples' internal order.
  • Common primers can be added by our staff free of charge upon request.

Plasmids

We also accept E.coli strains instead of purified plasmid DNA. We in vitro amplify plasmids from bacteria for DNA sequencing.

PCR-products

We purify PCR products from primers and dNTP using Exonuclease and gel filtration.

PCR Purification

Pricing

  • We guarantee competitive prices for both Non-profit and private sector organizations.
  • Please call in for a quote.

We purify PCR products from primer and dNTP by using exonuclease and gel filtration columns.

PCR products should have a single distinct band on agarose gel, otherwise, gel purification should be pursued.